本研究针对CRISPR-Cas9介导的同源定向修复(HDR)效率低下的难题，系统评估了DNA模板变性、RAD52蛋白辅助及5'-末端修饰等多种策略对Nup93条件性基因敲除(cKO)小鼠模型构建的影响。研究发现：变性单链DNA(ssDNA)模板可显著提升精确编辑效率并减少头尾串联重复；RAD52蛋白 ...

Cas9 is directed to its target within the DNA sequence through the use of the guide RNA. A specific sequence of DNA that is between two and five nucleotides in length must align with the 3’ end of the ...

本文报道了利用DNA-free CRISPR/Cas9技术对淀粉马铃薯品种"Kuras"中甾体糖生物碱（SGA）生物合成通路的关键基因进行定向编辑，成功获得SGA含量显著降低的突变体。研究靶向胆固醇合成前（SMO1-L、DWF1-L、DWF7-L）及合成后（16DOX、CYP88B1、TAMiso2）的6个关键基因，通过 ...

Researchers have now used a gene-drive system to suppress an important agricultural pest. Researchers have developed a "homing gene drive system" based on CRISPR/Cas9 that could be used to suppress ...

WEST LAFAYETTE, Ind. — More than a decade ago, scientists harnessed a bacterial molecular machine that identifies and cuts specific sections of DNA, revolutionizing the ability to edit genes and ...

Crispr Therapeutics, a leader in CRISPR/Cas9 biotechnology, recently received FDA and EMA approval for Casgevy, the first CRISPR/Cas9-based treatment for TDT and SCD. The partnership with Vertex ...

Whilst genome editing with CRISPR-Cas9 is effective, it runs the risk of off-target effects and excessive mutations, which can lead to toxicity. To reduce the likelihood of these occurring, ...

Scientists introduced CRISPR to the world as a gene-editing tool in summer 2012, when landmark papers from two independent groups demonstrated how the system could be wielded to make cuts in DNA. Now, ...

An exon-skipping technique using dual single-guide RNA/Cas9 ribonucleoproteins targeted at 3 novel COL7A1 exons with pathogenic heterozygous mutations achieved exon deletion rates of up to 95%.